S5E Figure

S5E Fig. AP-1 and NF-κB may be more active in IFN-β high cells. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) to block IFN-β paracrine signaling. RNA was extracted from BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs based on tdTomato expression (tdTomato+ and -). mRNA levels of targets of AP-1 and NF-κB – IL-6 and TNFAIP3 – were measured by RT-qPCR, normalized to 18S rRNA, and plotted relative to the bulk lytic+casp-i+anti-IFN Abs sample. ns = p>0.05, * = p