Transcriptomic analysis of HES induced [E(spl)mγ and dpn; DM] Drosophila larval primary CNS tumours vs HES allograft tumours in w1118 host flies

The goal of this project was to compare NGS derived transciptional profiles of primary larval CNS tumours and allograft tumours from two stages (first-T0 and forth-T3) in Drosophila that are induced by overactivation of two HES genes [E(spl)mγ and dpn;DM] in order to decipher if these two Hes genes control similar or different sets of genes as primary hypeplasias transition to malignant tumours. The results of this study show that overactivation of DM continues to negatively regulate neuronal differentiation genes as the DM hyperplasias become more malignant and that genes related to stress and metabolsim are upregulated mRNA profiles from FACS sorted, GFP positive Drosophila larval CNS DM tumours vs the first (T0) allograft DM tumours vs the forth (T3) serial allograft DM tumours