RNA-seq on SIP+-treated mating type minus cultures of Seminavis robusta

In order to obtain a global view on the transcriptional changes induced by the sex-inducing pheromone released by Seminavis robusta mating type plus cells (SIP+), an RNA-seq experiment was conducted. Therefore, purified SIP+ from RP-UPLC/MS was added to MT- cultures, 15 min before light onset after a prolonged dark period. MT- cultures without extract addition constituted the control conditions. Samples were taken after 15 min, 1 h and 3 h of illumination. An additional, non-treated MT- culture was harvested before illumination.