Repository of raw qPCR data to assess Sin Nombre hantavirus presence in lung tissues and replication in vitro

Background: Orthohantaviruses are negative-sense RNA viruses that can cause hantavirus cardiopulmonary syndrome (HCPS) in humans. In the United States, Sin Nombre orthohantavirus (SNV) is the primary cause of HCPS, with a fatality rate of 36% and most cases occuring in the southwestern states. The western deer mouse, Peromyscus sonoriensis, is the primary reservoir for SNV; however, it remains unclear if alternative reservoirs exist.
Results: We conducted an extensive survey of SNV genetic prevalence in wild-caught small mammal communities throughout New Mexico and observed that 27% of all animals were positive for SNV. Through longitudinal trapping at a site of patient exposure, we found that SNV circulates at a high rate in multiple species over time. Furthermore, we isolated live SNV from tissues and feces from multiple small mammal species, demonstrating infectious virus in alternative and novel reservoirs.
Significance: Altogether, this work shows that SNV is widely prevalent and p..., , , # Repository of raw qPCR data to assess Sin Nombre hantavirus presence in lung tissues and replication in vitro

[https://doi.org/10.5061/dryad.2547d7x2c](https://doi.org/10.5061/dryad.2547d7x2c)

## Description of the data and file structure

PCR data assessing Sin Nombre hantavirus prevalence in small mammal tissue samples.  Each tab lists the figure it is representing.

The data are RNA copies/mL from various rodent samples.  Each tab in the spreadsheet refers to the data used in the listed figure panel in the manuscript.  Some columns contain empty cells because there are more animals in some experiments compared to others, due to different trap rates

Figures 1-5:  Lung tissue was acquired and RNA was isolated and converted to cDNA as listed in the methods section.  Quantitative PCR was conducted using primer/probe sets specific for Sin Nombre hantavirus.  Shown are copies of viral RNA/mL.

Figures 6 and Supplemental Figure 1.  Tissues from hantavirus-positive mice were homogenized...