The shared PPARa/? target genes regulate brown adipocyte thermogenic function (ChIP-Seq)

We report the application of sequencing technology for high-throughput profiling of PPARa/? in mammalian cells. By obtaining over 3 billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse brown adipose tissue. We find that PPARa binds to a subset of PPAR? sites, suggesting a potential redundancy in which PPARa function may not be necessary in brown adipose tissue.While PPARa may not itself be necessary for BAT function, shared targets regulation by PPARa and PPAR? may nonetheless reveal relevant biology in this tissue.This study provides a framework for the application of comprehensive chromatin profiling towards characterization of PPARa/? regulation in mouse brown adipose tissue. Overall design: Examination of 2 different transcription factors in 2 cell types.