Ethanol exposure reveals persistent and categorizable astrocyte calcium response types and associated gene signatures. Ethanol exposure reveals persistent and categorizable astrocyte calcium response types and associated gene signatures

Various EtOH doses increased intracellular calcium in a subset of astrocytes, identifying three cellular response types categorized by response waveform properties S- (sustained), T- (transient), or M- (Mixed), along with non-responsive (NR) astrocytes. By single-cell RNA-seq analysis of astrocytes from the different response types, we report type-enriched discriminatory gene expression signatures and provide a basis for identifying the relationship between susceptibility to EtOH effects in the astrocyte subgroups and corresponding measurable markers of calcium signal and gene expression. Overall design: Single-cell RNA-seq for primarily cultured astrocytes from cortex of single C57BL/6 mouse pups. In total, there are 22 cells including 5 No Response (NR), 6 S-type, 6 T-type and 5 M-type astrocytes.