Source data: PolyPR interaction with nuclear transport components

The disruption of nucleocytoplasmic transport (NCT) is an important mechanism in neurodegenerative diseases. In the case of C9orf72-ALS, trafficking of macromolecules through the nuclear pore complex (NPC) might get frustrated by the binding of C9orf72-translated arginine-containing dipeptide repeat proteins (R-DPRs) to the Kapβ family of nuclear transport receptors. Beside Kapβs, several other types of transport components have been linked to NCT impairments in R-DPRs expressed cells, but the molecular origin of these observations has not been clarified. Here, we adopt a coarse-grained molecular dynamics model at amino-acid resolution to study the direct interaction between polyPR, the most toxic DPR, and various nuclear transport components to elucidate the binding mechanisms and provide a complete picture of potential polyPR-mediated NCT defects. We found polyPR to directly bind to several isoforms of the Impα family, CAS (the specific exporter of Impα) and RanGAP. We observe no bind..., , , # Source data: PolyPR interaction with nuclear transport components

[https://doi.org/10.5061/dryad.f7m0cfz46](https://doi.org/10.5061/dryad.f7m0cfz46)

The dataset includes source data files used to generate all figures in the manuscript titled \"C9orf72 polyPR directly binds to various nuclear transport components\" [https://doi.org/10.7554/eLife.89694.2](https://doi.org/10.7554/eLife.89694.2). The Excel files and sheet titles follow the same naming convention used in the manuscript to maintain consistency.

## Description of the data and file structure

Data for each figure is provided in Excel files, with separate sheets for each figure panel.

### Data and file overview

#### figure2.xlsx:

Source data for **figure 2** and **figure 2 figure supplements** in the manuscript.

##### Sheets:

*NCPR: net charge per residue, M: Dipole moment, Rg: radius of gyration

1- fig2a-100mM/200mM: Normalized number of contacts between polyPR ( with 7,20, and 50 repeat units) and different transport ...