Differential roles of Stella in the modulation of DNA methylation during oocyte and zygotic development. Differential roles of Stella in the modulation of DNA methylation during oocyte and zygotic development

In order to obtain genome-wide profiles, base-resolution methylomes of oocytes and zygotes were generated using the bisulfite sequencing (BS-seq) method for small samples. We find that global loss of DNA methylation during zygotic development in HFD mice. A total of 412 DMRs were identified, of which 294 were hypomethylated (hypo-DMRs; 71.4%) and 118 were hypermethylated (hyper-DMRs; 28.6%) (Fig. 6A and 6B), showing a predominance of hypo-DMRs. Furthermore, we show that hyper-DMRs are significantly depleted from CGI, but enriched in short interspersed elements (SINEs) and non-CGI regions. Strikingly, hypo-DMRs are specifically depleted from SINEs, with a concurrent enrichment in DNA transposons. Overall design: Examination of DNA methylome in oocyte and zygote from Stella∆ and WT