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Inference of B cell clonality and function from single cell RNA seq data

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NIAID Data Ecosystem2026-03-10 收录
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https://www.omicsdi.org/dataset/ega/EGAS00001002963
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cDNA libraries for RNA-seq will be prepared for sequencing. cDNA will be created following the Smart-Seq2 protocol, and a part of the cDNA from each cell will be used for preparation of RNA-seq libraries using the Nextera XT kit. The other part of the cDNA will be used as template for verification of the computationally recovered immunoglobulin sequences from the RNA-seq dataset by using a targeted PCR approach to generate a multiplexed sequencing libraryEGA study EGAS00001002963
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2018-08-01
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