Relative activity of lipases cloned from Anaerovibrio lipolyticus 5ST after incubation for 30 min with different metal ions.
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The enzymes were incubated 30 min in 50 mM MES buffer (pH 6.5) with the metal ions at 5 mM final concentration; the residual activities were measured with a standard assay against ρ-nitrophenyl caprylate (C8). The activity of the enzyme not incubated with ions was defined as 100%. Control activities (inincubated) were 337, 296 and 270 U·mg−1 protein for alipA, alipBss and alipC respectively).
创建时间：
2015-12-02
