Gossypium raimondii Transcriptome or Gene expression

The sequencing was performed on 454/Roche FLX technology using normalized cDNA libraries prepared from young leaves, roots, bolls, ovules, and fibers of G. raimondii. Reads from two independent sequencing samples were combined to assemble contigs. The combined sequencing analysis using 1,464,815 reads from D-genome resulted in 65,542 contigs for D-genome. The majority of contigs (~70%) ranged from 200 to 1,000-bp.This large set of D-genome ESTs will be valuable resources for gene expression study and crop improvement in cultivated allotetraploid cotton.