PSE115_PSM1063_mouse

Placentas from naturally-mated crosses of C57Bl/6J mice on embryonic day 17.5 were cut into thick slices. A scalpel blade was used to isolate the blood-red labyrin th tissue from the more poorly vascularized, and hence paler, spongiotrophoblast tissue, and to remove the superficial chorionic vasculature. From each litter, of the colle cted labyrinth tissues were set aside for RNA extraction and microarray analysis and for cellular fractionation and proteomic analysis, as recently described (Kislinger et al., 2006).