CDH1 knockout in mice

Employing Cre/loxP technology, a mouse model of liver specific Cdh1 (E-cadherin) depletion was created (fl/fl | +/wt). Previously, the mice had already been compared to littermates with normal Ecadherin levels (fl/fl | wt/wt), using a broad range of laboratory methods (Serum parameters, long-term weight analysis, histology, western blot, etc.). To analyze the effect of Cdh1 (E-Cadherin) depletion on the liver homeostasis on the RNA-level over the course of 3 time-points, liver tissue was aquired from mice groups aged 1 week, 3 weeks and 6 weeks. Mice with liver-specific Cdh1 depletion were compared to wildtype-like littermates. 1 week: n=2+2; 3 weeks: n=3+3; 6 weeks: n=3+3. Samples (mice) were not paired.本研究采用Cre/loxP重组酶系统，构建了肝脏特异性敲除Cdh1（E-钙粘蛋白，E-cadherin）的小鼠模型（基因型为fl/fl | +/wt）。此前，研究团队已通过多种实验技术（血清参数检测、长期体重分析、组织病理学、蛋白质免疫印迹等），将该模型小鼠与E-钙粘蛋白表达正常的同窝野生型小鼠（基因型为fl/fl | wt/wt）开展了对照分析。为探究Cdh1（E-钙粘蛋白）敲除在三个时间节点下对肝脏稳态的RNA水平影响，本研究从1周龄、3周龄及6周龄的三组小鼠中采集了肝脏组织样本。本研究将肝脏特异性Cdh1敲除小鼠与野生型同窝对照小鼠进行分组对比。各组样本量分别为：1周龄组n=2+2；3周龄组n=3+3；6周龄组n=3+3。所有小鼠样本均未采用配对实验设计。