Inverse PCR sequences

AACA4F-AACA4RI (REACTION AACA4F): An inverse PCR reaction was performed with primers AACAF and AACA4RI and the sequencing reaction was performed with primer AACA4F. This sequencing revealed the excised, circular form with the configuration “aacA4-gcu14” AACA4F-AACA4RI (REACTION AACA4RI): An inverse PCR reaction was performed with primers AACAF and AACA4RI and the sequencing reaction was performed with primer AACA4RI. This sequencing revealed the excised, circular form with the configuration “aacA4-gcu14” GESFSQ-GESR (REACTION GESFSQ): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer GESFSQ. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.GESFSQ-GESR (REACTION GESR): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer GESR. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.GESFSQ-GESR (REACTION AACA4F): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer AACA4F. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.GESFSQ-GESR (REACTION AACA4FI): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer AACA4FI. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.GESFSQ-GESR (REACTION AACA4R): An inverse PCR reaction was performed with primers GESFSQ and GESR and the sequencing reaction was performed with primer AACA4R. This sequencing revealed the excised, circular form with the full length configuration “gcu14-GES-1/aacA4”.Orf126 menor: An inverse PCR reaction was performed with primers GCU FSQ and GCU RSQ and the sequencing reaction was performed with primer GCU FSQ. This sequencing revealed the excised, circular form with the configuration “gcu14”.