Female fusome volumes and validation by manual reconstructions.

Fusome volumes and fusome volume fractions are shown from measured automated reconstructions from ilastik and MATLAB. The list comprises measured volumes from 2-cell (n = 12), 4-cell (n = 12), 8-cell (n = 6), and 16-cell (n = 11) cysts, along with estimated volume fractions at each stage given the estimate of α = 0.7, β = 0.5 for the values of the model parameters. In addition, manual volume reconstructions on a randomly chosen group of samples (n = 8) using thresholding in FIJI shows a high agreement between manual and automated fusome volume reconstruction in 3D. (XLSX)