Composition of media.
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Except in columns labelled pH and osmolality, values are in mM. pH of all media was adjusted with NaOH or HCl without changing final [Na+] or [Cl−] as tested.
a Numbers in column refer to [H4SiO4] or [H4GeO4]. Note that Si is a ubiquitous trace contaminant so that its concentration in the solutions not added with H4SiO4 was actually ~2–3 μM rather than 0.
b pH was adjusted at either 6.4, 7.4 or 8.4 in 80 mM acetate (to change both extracellular and intracellular pH) or in 80 mM gluconate (to change extracellular pH only [18]).
Abbreviations: Bic, HCO3−; HEP, HEPES; NMG, N-Methyl-D-glucamine; Ace, acetate; Glu, gluconate; Suc, sucrose; Osm, osmolality (mOsM).
Composition of media.
创建时间：
2015-08-27
