five

EEL mouse sagittal 440 genes RNA spatial data

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Figshare2022-09-22 更新2026-04-08 收录
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https://figshare.com/articles/dataset/EEL_mouse_sagittal_440_gene_RNA_spatial_data/20324820/3
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<strong>Raw RNA locations of a sagittal mouse brain section produced by EEL FISH for 440 genes.</strong> RNA files are in the .parquet format which can be opened with FISHscale (https://github.com/linnarsson-lab/FISHscale) or any other parquet file reader (https://arrow.apache.org/docs/index.html) <br> <strong>RNA .parquet files</strong> Position and gene label for all RNA molecules. <br> "c_px_microscope_stitched" contains X coordinates. "r_px_microscope_stitched" contians Y coordinates. The unit are pixels with a size of 0.18 micrometer. Multiply by 0.18 to get um scale. <br> "c_transformed" contains X coordinates. "r_transformed" contains Y coordinates. The units are pixels with a size of 0.27 micrometer. Multiply by 0.27 to get um scale. These coordinates match the coordinates in the single cell .loom file. <br> "hamming_distance" is the hamming distance divided by the barcode length (16). The closer to 0 the better the barcode. <br> <strong>Tissue polygon .csv file</strong> The .csv flie contains a polygon of the tissue area to clip RNA molecules outside. The unit size is in micrometer. <br> <strong>Gene colors .pkl file</strong> Pickled Python dictionary with gene colors used in the paper.
提供机构:
Borm, Lars
创建时间:
2022-09-20
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