Single cell transcriptomic analysis of aging hair follicles

Tissue stem cells are required for homeostasis and wound repair throughout the entire lifespan. Multiple mechanisms have been extensively studied for the maintenance of stem cell. Here we use single cell RNAseq to study the stem cell maintenance during aging. We sought out to compare the gene expression changes in different cell lineages between control, Foxc1/Nfatc1 double knockout mice and aging mice. Overall design: For single cell RNAseq, the epidermal cells were sorted such that the interfollicular epidermis and hair follicle cells were at the ratio of 1:3. Around 5k cells were collected and used for the assay. We followed the 10xgenomics single cell RNAseq protocol version3.