Effect of skn-1 RNAi on Pgst-4::GFP induction by plumbagin.

Animals (CL2166) were raised from embryo to adulthood on HT115 bacteria containing either the L4440 RNAi vector or a plasmid inducing skn-1 RNAi. Adults were treated for two days with plumbagin or DMSO vehicle control before GFP visualization.∧ AMPI represents the average whole worm background subtracted mean pixel intensity for the treatment group.†AMPI ratio for treatment relative to the L4440 vehicle (DMSO) control.T-test compares the AMPI of each worm in the treatment group with those of the control (0 µM plumbagin, L4440) in that experiment.