Enhanced force generation by smooth muscle myosin in vitro.
收藏PubMed Central1994-01-04 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC42914/
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资源简介：
To determine whether the apparent enhanced force-generating capabilities of smooth muscle relative to skeletal muscle are inherent to the myosin cross-bridge, the isometric steady-state force produced by myosin in the in vitro motility assay was measured. In this assay, myosin adhered to a glass surface pulls on an actin filament that is attached to an ultracompliant (50-200 nm/pN) glass microneedle. The number of myosin cross-bridge heads able to interact with a length of actin filament was estimated by measuring the density of biochemically active myosin adhered to the surface; with this estimate, the average force per cross-bridge head of smooth and skeletal muscle myosins is 0.6 pN and 0.2 pN, respectively. Surprisingly, smooth muscle myosin generates approximately three times greater average force per cross-bridge head than does skeletal muscle myosin. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1994-01-04
