NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence [ChIP-Seq]

We describe the accessible chormatin landscape in RAS-induced (RIS) and NOTCH induced senescence (NIS) using ATAC-seq. By expressing active NOTCH (N1ICD) in the context of RIS, we find that N1ICD antagonizes the formation of accessible regions in RIS. By performing co-cultures, we demonstrate that cells expressing a NOTCH1 ligand, JAGGED1, can antagonize the formation of RIS specific accessible regions. Many of these accessible regions form at enhancers, characterised using ChIP-seq against H3K4me1 and H3K27Ac Overall design: Mapping of H3K4me1 and H3K27Ac using ChIP-Seq in IMR90 ER:HRAS(G12V) cells treated with 4OHT for 6 days or untreated