Transcriptome sequencing and microRNA–mRNA regulatory network construction in the lens from a Na2Se03-induced Sprague Dawley rat cataract model [miRNA]

To explore the regulatory mechanism of age-related cataract (ARC) formation and progression，we construct sodium selenite-induced rat cataract model and performed the high-throughput RNA sequencing (HTS) technology to identify the mRNA and miRNA expression profiles of the lens from Na2Se03-induced and saline - injected Sprague Dawley rats.为探究年龄相关性白内障（age-related cataract, ARC）发生与进展的调控机制，本研究构建了亚硒酸钠诱导的大鼠白内障模型，并采用高通量RNA测序（high-throughput RNA sequencing, HTS）技术，对亚硒酸钠（Na₂SeO₃）诱导组与生理盐水注射组斯普拉-道来（Sprague Dawley, SD）大鼠的晶状体mRNA及miRNA表达谱进行鉴定。