Syp-GFP distribution patterns in developing and mature GCs.

(A) Representative confocal image of a labeled immature cerebellar GC from the mouse with the following genotype: ZtTA/wt; TRE-Bi-SG-T/wt; β-actin-CreER/wt, with tamoxifen administered at P7, and dissected at P15. In the merged panel Syp-GFP appears yellow, while tdT (red) fills the processes. (B) Quantification of Syp-GFP distribution in axonal and dendritic regions in mature and immature GCs showing that Syp-GFP becomes enriched in the axon after maturation. The graph shows an average for 10 cells for each condition. For mature cells, the standard deviation is ±11%; for immature cells, the standard deviation is ±7%. Two animals were used for analysis.