DHPSFU: a Fiji plug-in for fast and accurate double helix-PSF 3D single-molecule localisation microscopy

The dataset is linked to Zhang et al, "DHPSFU: a Fiji plug-in for fast and accurate double helix-PSF 3D single-molecule localisation microscopy", Scientific Reports 2025.

The repository contains simulated double-helix PSF imaging data alongside lists of ground truth localisations, as well as a real double-helix PSF imaging dataset of WGA-stained T-cell surface. For one simulated dataset, all data processing using PeakFit and DHPSFU algorithms are included as an example. For the real data, the DHPSFU output and the post-processing steps are also outlined.

The data is uploaded as three ZIP files, corresponding to the real dataset and the two simulated datasets. Please refer to the README file for detailed descriptions of the files, and to the publication for more details regarding data acquisition and analysis.