High-throughput detection of M. agalactiae mutants unable to grow on cultured cells.

aThe cut-off value is the number of colonies counted on solid medium following 3 days co-cultivation of M. agalactiae knockout mutants with cells. Titers in parentheses indicate the predicted titers in the co-culture.bNumber of M. agalactiae growth deficient mutants selected on goat mammary epithelial cells (TIGMEC), goat embryonic fibroblast cells (TIGEF), and HeLa cells (HeLa).cPercentage of growth deficient mutants selected from the mutant library.