Region 3 of the NB-ARC domain contributed to the sensor-helper compatibility.

(A) Cell death assays of chimeric NRC3 variants designed for investigating regions of the NB-ARC domain that contribute to sensor-helper compatibility. The variants were co-expressed with Rpi-blb2/AVRblb2 or Pto/AvrPto in nrc2/3/4_KO N. benthamiana. Auto-activity analysis was done by expressing NRC3 variants alone in WT N. benthamiana. The dot plots represent cell death intensity quantified by UVP ChemStudio PLUS at 6 dpi. The line in the boxplots represents the medium, the box edges represent the 25th and 75th percentiles, and the whiskers extend to the most extreme data points no more than 1.5x of the interquartile range. Statistical differences were examined using Dunn’s test (p < 0.05). (B) Protein accumulation of chimeric NRC3 variants tested in (A). NRC3 variants were transiently expressed in WT N. benthamiana. The proteins were extracted from leaf samples at 2 dpi and the NRC3 protein accumulations were detected by α-myc antibody. SimplyBlue SafeStain-staining of Rubisco was used as the loading control.
(JPF)