Hauturu temperate rainforest soil environmental sequencing using targeted loci (16S, 18S, ITS, COI and trnL). soil metagenome

From 20 soil samples we amplified DNA from five molecular markers (16S, 18S, ITS, COI and trnL) using targeted-locus next generation sequencing. Our aim was to evaluate a suite of DNA markers coupled with next generation sequencing (NGS) that span the tree of life and compare these data to traditional biodiversity monitoring tools, including mist-netting and call counts for birds, vegetation surveys, and invertebrate collection with DNA barcoding. Our sampling regime consisted of ten 20x20 metre plots, each comprising sixteen 5x5 metre sub-plots along a 700 metre elevational gradient on a temperate island forest ecosystem (Hauturu, New Zealand).