SPARKEL depletion time course proteome

Apicomplexan parasites are ubiquitous pathogens of humans. Toxoplasma gondii, a model apicomplexan, possesses a divergent ortholog of mammalian PDK1. This ortholog, TgSPARK, is essential for the ability of parasites to invade and exit their host. We discovered an interactor of SPARK, an elogin-like protein that we termed SPARKEL. Here, we conduct a quantitative mass spectrometry experiment to measure changes to the parasite proteome following 0, 3, 8, and 24 hours of conditional SPARKEL depletion.