Transcription attenuation in synthetic promoters in nonoverlapping tandem formation

Closely spaced promoters are ubiquitous in prokaryotic and eukaryotic genomes. How their structure and dynamics relate remains unclear, particularly for tandem formations. To study their transcriptional interference, we engineered two pairs and one trio of synthetic promoters in nonoverlapping, tandem formation, in single-copy plasmids transformed into Escherichia coli cells. From in vivo measurements, we found that these promoters in tandem formation can have attenuated transcription rates. The attenuation strength can be widely fine-tuned by the promoters’ positioning, natural regulatory mechanisms, and other factors, including the antibiotic rifampicin, which is known to hamper RNAP promoter escape. From this, and supported by in silico models, we concluded that the attenuation in these constructs emerges from premature terminations generated by collisions between RNAPs elongating from upstream promoters and RNAPs occupying downstream promoters. Moreover, we found that these collisio..., , , This READ ME.md file was generated on 10-04-2024.

# Transcription Attenuation in Synthetic Promoters in Nonoverlapping Tandem Formation

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### 1. Principal Investigator contact information:
- Name: Andre S. Ribeiro
- Institution: Tampere University
- Email: [e-mail]

### 2. Description of the dataset:
This dataset contains flow cytometry and spectrophotometry data reagarding synthetic cosntructs of individual promoters (PLacO3O1, PtetA and PBAD) and of those promoters when in tandem formation (PLacO3O1-PtetA, PtetA-PLacO3O1 and PtetA-PBAD-PLacO3O1).

## Description of the data and file structure

### 1. File list:
##### Flow Cytometry: This folder contains all flow cytometry data used in the project.
**Note**: Unless stated otherwise, in the subfolders, there are 3 CSV files labelled \"Sample 1\", \"Sample 2\", and \"Sample 3\" that represent the measurements of 3 biological replicates in a given condition.

- 01 Single point measurements: Contains data measured by ...