Oxidative dealkylation of 1-EtA damaged DNA by ABH3

ALKBH3, a homolog of E.coli AlkB (Trewick et al. 2002), removes the ethyl group from 1-ethyladenine (1-etA) in a reaction dependent on alpha-ketoglutarate, oxygen and Fe2+. ALKBH3 directly reverses alkylating damage of DNA in the form of 1-etA, releasing acetaldehyde (Duncan et al. 2002). The reversal of alkylating damage of dsDNA by ALKBH3 requires the presence of DNA helicase ASCC3, a component of the activating signal co-activator complex (Dango et al. 2011). ALKBH3 can also repair methylated RNA (Aas et al. 2003).

ALKBH3，大肠杆菌AlkB同源物（Trewick等，2002年），能够在依赖于α-酮戊二酸、氧气和Fe2+的反应中移除1-乙基腺嘌呤（1-etA）的乙基。ALKBH3能够直接逆转以1-etA形式存在的DNA烷基化损伤，释放乙醛（Duncan等，2002年）。ALKBH3逆转双链DNA烷基化损伤的过程需要DNA解旋酶ASCC3的存在，ASCC3是激活信号共激活复合体（Dango等，2011年）的组成部分。此外，ALKBH3还能够修复甲基化的RNA（Aas等，2003年）。