Fluorescence emission spectra of Laurdan in phase-separated solid-supported lipid bilayer excited with azimuthally, radially and linearly polarized beams

Fluorescence emission spectra of Laurdan in liquid-ordered and liquid-disordered phases in solid-supported lipid bilayer exhibiting coexisting phases, excited with azimuthally, radially and linearly polarized laser beams. Lipid bilayer was composed of an equimolar mixture of 1,2-dimyristoleoyl-sn-glycero-3-phosphocholine, cholesterol, and egg yolk sphingomyelin. Mica was used as a solid support. Laurdan was excited with a picosecond laser diode with λ = 375 nm (PicoQuant). Spectra were collected with a spectrograph Kymera 328I-C (Andor) equipped with an EMCCD camera iXon Ultra 888 (Andor).