In Vivo and In Vitro Dynamics of Undifferentiated Embryonic Cell Transcription Factor 1. Mus musculus

Pluripotent stem cells retain the ability differentiate into the three germ layers and germ line. As a result, there is a major interest in characterizing regulators that establish and maintain pluripotency. The network of transcription factors continues to expand in complexity, and one factor, Undifferentiated embryonic cell Transcription Factor 1 (Utf1), has recently moved more into the limelight. To facilitate the study of Utf1, we report the generation and characterization of two reporter lines that enable efficient tracking, mapping and purification of endogenous Utf1. In particular, we include a built-in biotinylation system into our targeted locus that allows efficient and reliable pulldown. We also use this reporter to show the dynamic regulation of Utf1 in distinct stem cell conditions and demonstrate its utility for reprogramming studies. The multi-purpose design of the reporter lines enables many directions of future study and should lead to a better understanding of Utf1’s diverse roles. Overall design: ChIP-Seq for endogenous and biotin tagged Utf1 in KH2 mouse embryonic stem cells