Gene expression analysis of macrophages exposed to LPS and LPS-coated beads

The aim of this study was to measure the response of alveolar macrophages (AM) and bone marrow-derived macrophages (BMDM) to LPS, LPS-coated beads, and PBS-coated beads Mice were exposed to LPS-coated beads or PBS-coated beads using a nebulizer. Alveolar macrophages were isolated 4 hours after exposure by broncholaveolar lavage and by fluorescence activated cell sorting using the following surface markers (CD45+, Zombie Violet-, CD3-/CD19-, Siglec F+, CD64+, FITC-bead+ or FITC-bead-). Bone marrow-derived macrophages were collected 4 hours after exposure to LPS-coated beads, PBS-coated beads, or soluble LPS by fluorescence activated cell sorting using the following surface markers (CD45+, Zombie Violet-, CD3-/CD19-, F4/80+, FITC-bead+ or FITC-bead-). Samples were analyzed by RNA-sequencing.