poly(A+) RNAs 3'-seq analysis in control and FUS KD (knock-down) samples

To understand the effect of FUS protein knock-down on poly(A+) RNAs profiles, we carried out control and FUS depletion (using synthetic siRNAs) experiments followed by poly(A+) RNA 3'-seq anlyais using lexogen kit (016.024)in HeLa cells Overall design: siRNAs targeting FUS was designed and transfected into Hela cells,knock-down efficiency was confirmed by western blots. 3'-seq analysis was performed using lexogen kit (016.024) si-control-U1-AMO: This experiment was designed and carried out to serve as positive control for 3'-seq under FUS knockdown condition Negative control siRNA and U1 AMO was transfected into Hela cells, 3'-seq was subsequently carried out to examine the polyadenylation site usage at the transcriptome level using a lexgen kit (Cat. 016.24)