iPS derived microglia like cells cultured with human neurons and astrocytes. iPS derived microglia like cells cultured with human neurons and astrocytes

In this article, Jose Polo and colleagues molecularly compare existing microglia differentiation methods from stem cells, generate a new dual fluorescent and enzymatic reporter tool to study microglia in vitro and in vivo and show that human astrocyte/neuron co-culture pushes the regulatory landscape of in vitro microglia-like cells to more closely resemble bona fide microglia. Overall design: RNA-seq experiment examination of iPS-derived microglia like cells cultured in presence (or not) of human astrocytes/neurons, or cholesterol