SIFT, Polyphen-2, PhyloP, LRT, Mutation Taster, and GERP++ are functional prediction scores in which increasing values indicate a probable mutation. ESP5400 and 100g2012feb are the allele frequency in
The mutation areas were amplified with PCR and the amplicons were sequenced on the Illumina MiSeq. The background error levels were between 0.01–0.5% and were estimated by quantifying errors in positi