HyperTRIBE uncovers SYNCRIP RNA binding activity in hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs). HyperTRIBE uncovers SYNCRIP RNA binding activity in hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs)

Identifying SYNCRIP RNA-binding targets in HSC and MPP populations, in order to uncover which direct targets influence SYNCRIP's role in maintaining HSCs self renewal capacity. Overall design: HSCs (Lin-, Sca1+, cKit+, CD150+ CD48-) and MPPs (Lin-, Sca1+, cKit+, all remaining CD150 CD48 cells) from C57BL/6 mice (The Jackson Laboratory, USA) were FACS sorted and transduced with virus expressing either empty vector (MSCV-IRES-GFP) or SYNCRIP-ADAR. 48hrs post transduction cells were sorted for GFP+ expression and used for RNA-seq.