Schistosoma mansoni ATAC-seq results for IGV (female and male worms with and without LSD1 inhibitor)

In this study, the anti-schistosomal activity of 39 <em>Homo sapiens</em> Lysine Specific Demethylase 1 (HsLSD1) inhibitors was investigated on parasitic life cycle stages associated with both definitive and intermediate host infection. Amongst this collection of small molecules, compound <strong>33</strong> was the most potent and reduced <em>ex vivo</em> viabilities of schistosomula, juveniles, miracidia and adults. At its sub-lethal concentration to adults (3.13 µM), compound <strong>33 </strong>also significantly impacted oviposition, ovarian as well as vitellarian architecture and gonadal/neoblast stem cell proliferation. ATAC-seq analysis of adults demonstrated that compound <strong>33</strong> significantly affected chromatin structure (intragenic regions &gt; intergenic regions), especially in genes differentially expressed in cell populations (e.g., germinal stem cells, hes2<em>⁺</em>stem cell progeny, S1 cells and late female germinal cells) linked to these <em>ex vivo</em> phenotypes. The data presented here allow for visualisation in IGV https://igv.org/app/ Produced in collaboration with IHPE.