Site-directed mutagenesis analysis of the role of selected active site residues on the steady-state kinetic parameters of NAD+ transformation catalyzed by bCD38.
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aConversion of NAD+ into cADPR, in percent of the reaction products,bcADPR was determined using the cycling assay, the other data were obtained using a radiometric HPLC assay with 14C-NAD+ as substrate.
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2015-12-02
