Inoculation diesel contaminated soil Metagenome

In order to cope with and mitigate climate change, the demand for rhizoremediation technology against climate change is increasing not only in the development of soil rhizoremediation but also in the development of nitrous oxide emission reduction. This study investigated the effect of Pseudomonas sp. inoculation on the nitrous oxide emission in the rhizoremediation process of oil contaminated soil. Two of Pseudomonas sp. , N2O-reducing bacteria, was isolated from the soil of the tall fescue and maize, respectively. In 59 pot soil experiment, Pseudomonas sp. M23 and Pseudomonas sp. TF716 was inoculated in diesel 10,000 ppm contaminated soil planted maize and tall fescue, and maize and tall fescue were planted without Pseudomonas sp. as a control group. In this study, M23 and TF716 showed high resistance to contaminated environment with high concentration of diesel, and improved the potential N2O reducing ability of soil and root growth of plants. In particular, the dominance of Pseudomonas and the potential N2O reducing ability in soils inoculated with TF716 and planted with tall fescue were significantly higher than other conditions. These results suggest that M23 and TF716 can be used effectively to reduce N2O emissions during rhizoremediation of oil-contaminated soil.