Unravelling the developmental roadmap towards human brown adipose tissue

We have developed and characterised functionally and molecularly a new chemically-defined protocol for the differentiation of human pluripotent stem cells (hPSCs) into brown adipocytes (BAs) that overcomes current limitationsThis protocol recapitulates step-by-step the physiological developmental path of human BAT.The BAs obtained expresses brown adipocyte and thermogenic markers, are insulin sensitive, and responsive to β-adrenergic stimuli.This new protocol is scalable, enabling the study of human BAs at early stages of development.