Enlargement of a coalesced tubulo-vesicular structure in adipocytes treated with ESCRT-III constructs.

The areas of enlarged vesicular structures, with visible lumens and positive for EGFP, GLUT4 and ubiquitin or the endosomal markers, were measured in pEGFP VPS4E235Q and in wild-type pEGFP-Vps4 transfected adipocytes (as described in the Materials and Methods section). Results are mean and SEM from 10 to 14 individual cells. *** pvs control vesicles). Only the large pixel areas from the enlarged structures could be accurately converted to μm2.