Independent confirmation of microarray data by real-time PCR.

Note.
*p<0.05.
**p≤0.01.
***p≤0.001.
HFFs were stimulated with 1000 particles/cell of entry-defective HSV-1 and changes in cellular gene expression were detected by real-time PCR at 6 hours post-inoculation. NF-κB responsive genes appear to be up-regulated by binding of either ΔgB or ΔgH virions to the cell. The increase in NF-κB-associated transcripts appears specific as not all targets were differentially regulated. A similar pattern of gene expression is seen for Jak/Stat pathway targets in that ΔgD virions appear to be unable to stimulate signalling whereas ΔgB and ΔgH binding can. MMP7 is only associated with the PI3 Kinase/Akt pathway and coupled with the up-regulation of BCL2, CCND1 and MYC, it would appear that binding may be sufficient to also stimulate this pathway, although not by virions lacking in gD. The Jak/Src pathway only contains two targets on the signalling specific microarray, one of which, BCL2, was associated with other pathways.