A high-throughput mutational scan of an acidic transcriptional activation domain

Name: A high-throughput mutational scan of an acidic transcriptional activation domain
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doi.org2025-01-22 收录

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Raw data for Staller et al. 2018 A high-throughput mutational scan of an acidic transcriptional activation domain Max V. Staller1, 2, Alex S. Holehouse3,4, Devjanee Swain-Lenz1,2,5, Rahul K. Das3,4,6, Rohit V. Pappu3,4, and Barak A. Cohen1, 2, * 1Edison Family Center for Genome Sciences and Systems Biology, Washington University in St. Louis School of Medicine, Saint Louis, MO, 63110 2Department of Genetics, Washington University in St. Louis School of Medicine, Saint Louis, MO, 63110 3Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO, 63130 4Center for Biological Systems Engineering, Washington University in St. Louis, Saint Louis, MO, 63130 5Present address: Department of Biology, Duke University, Durham, NC, 27708 6Present address: GNS Healthcare Inc., Cambridge, MA, 02139 * Corresponding Author and Lead contact: cohen@wustl.edu Files include: raw sequencing reads for replicate 1 (Sort 11) raw sequencing reads for replicate 2 (Sort 12 ) raw sequencing reads for replicate 1 of amino acid starvation condition (Sort 14) for calculating induction raw sequencing reads for mCherry only sorting (sort 15A) File of designed mutants (DNA sequence) (DNA_Seqs_GCN4_Array.txt). raw sequencing reads for paired end sequencing to look for mutations in the library (Sort11AD-BC R1 and R2) Key to barcodes: Sort_Bin 5’ inline barcode 3’ adaptor barcode S11_1 GCTCGAT IND71 S11_2 TAGACTAT IND71 S11_3 CGCTACCCT IND71 S11_4 ATAGTGGACA IND71 S11_5 GCTCGAT IND72 S11_6 TAGACTAT IND72 S11_7 CGCTACCCT IND72 S11_8 ATAGTGGACA IND72 S12_1 GCTCGAT IND69 S12_2 TAGACTAT IND69 S12_3 CGCTACCCT IND69 S12_4 ATAGTGGACA IND69 S12_5 GCTCGAT IND70 S12_6 TAGACTAT IND70 S12_7 CGCTACCCT IND70 S12_8 ATAGTGGACA IND70 S14_1 GCTCGAT IND69 S14_2 TAGACTAT IND69 S14_3 CGCTACCCT IND69 S14_4 ATAGTGGACA IND69 S14_5 GCTCGAT IND70 S14_6 TAGACTAT IND70 S14_7 CGCTACCCT IND70 S14_8 ATAGTGGACA IND70 S15_1 GCTCGAT IND69 S15_2 TAGACTAT IND69 S15_3 CGCTACCCT IND69 S15_4 ATAGTGGACA IND69 S15_5 GCTCGAT IND70 S15_6 TAGACTAT IND70 S15_7 CGCTACCCT IND70 S15_8 ATAGTGGACA IND70

原始数据集，用于Staller等人2018年的研究：对酸性转录激活域进行高通量突变扫描。 Max V. Staller（1, 2）、Alex S. Holehouse（3, 4）、Devjanee Swain-Lenz（1, 2, 5）、Rahul K. Das（3, 4, 6）、Rohit V. Pappu（3, 4）和Barak A. Cohen（1, 2）* 1爱迪生基因组科学和系统生物学中心，圣路易斯华盛顿大学医学院，密苏里州圣路易斯，63110 2遗传学系，圣路易斯华盛顿大学医学院，密苏里州圣路易斯，63110 3生物医学工程系，圣路易斯华盛顿大学，密苏里州圣路易斯，63130 4生物系统工程技术中心，圣路易斯华盛顿大学，密苏里州圣路易斯，63130 5现工作单位：杜克大学生物学系，北卡罗来纳州达勒姆，27708 6现工作单位：GNS Healthcare Inc.，马萨诸塞州剑桥，02139 * 联系作者和主要联系人：cohen@wustl.edu 文件包括： 1. 复制1（排序11）的原始测序读数 2. 复制2（排序12）的原始测序读数 3. 氨基酸饥饿条件下复制1（排序14）的原始测序读数，用于计算诱导 4. 仅mCherry排序（排序15A）的原始测序读数 5. 设计的突变体文件（DNA序列）（DNA_Seqs_GCN4_Array.txt） 6. 对库中突变进行配对末端测序的原始测序读数（Sort11AD-BC R1和R2）条形码键： Sort_Bin 5’内联条形码 3’适配器条形码 S11_1 GCTCGAT IND71 S11_2 TAGACTAT IND71 S11_3 CGCTACCCT IND71 S11_4 ATAGTGGACA IND71 S11_5 GCTCGAT IND72 S11_6 TAGACTAT IND72 S11_7 CGCTACCCT IND72 S11_8 ATAGTGGACA IND72 S12_1 GCTCGAT IND69 S12_2 TAGACTAT IND69 S12_3 CGCTACCCT IND69 S12_4 ATAGTGGACA IND69 S12_5 GCTCGAT IND70 S12_6 TAGACTAT IND70 S12_7 CGCTACCCT IND70 S12_8 ATAGTGGACA IND70 S14_1 GCTCGAT IND69 S14_2 TAGACTAT IND69 S14_3 CGCTACCCT IND69 S14_4 ATAGTGGACA IND69 S14_5 GCTCGAT IND70 S14_6 TAGACTAT IND70 S14_7 CGCTACCCT IND70 S14_8 ATAGTGGACA IND70 S15_1 GCTCGAT IND69 S15_2 TAGACTAT IND69 S15_3 CGCTACCCT IND69 S15_4 ATAGTGGACA IND69 S15_5 GCTCGAT IND70 S15_6 TAGACTAT IND70 S15_7 CGCTACCCT IND70 S15_8 ATAGTGGACA IND70

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