NGS sequencing data on 22 different species or ssp Raw sequence reads. Anaxagorea crassipetala, Annickia affinis, Anonidium mannii, Coffea canephora, Digitaria abyssinica, Digitaria acuminatissima, Digitaria adscendens=ciliaris, Digitaria argillacea, Digitaria aristulata, Digitaria exilis, Friesodielsia enghiana, Greenwayodendron suaveolens, Monocarpia kalimantanensis, Monodora myristica, Neo-uvaria acuminatissima, Oryza glaberrima, Oryza sativa, Oryza sativa spp indica, Oryza sativa spp japonica, Cenchrus americanus , Cenchrus americanus spp monodii, Podococcus acaulis, Podococcus barteri

We assess multiplexed target enrichment via hybridization-based capture follow by next generation sequencing (NGS) in different species. We used species with different genome size and enrichment was done for differents target size. We show that high multiplexing level in a single hybridization step is an interesting strategy.