To verify whether the CcpA gene was successfully deleted, we designed the primer for PCR. The primer was designed across the upstream and downstream sequences of the CcpA gene, with a size of about 18
Verification of deletion of the MR from BMDCs. Western blot for the MR using an antibody raised to the MR N terminus. A band was detected in the wild type sample of the correct size for MR (Lanes 1 an
