Biological characteristics of gene expression features in pancreatic cancer cell induced by proton and x-ray irradiation I

The aim of the study is to disclose the distinct alteration of biological characteristics of gene expression features in pancreas cancer cell, MIAPaCa-2, irradiated by proton and x-ray. The survived MIAPaCa-2 cells after irradiation with proton and x-ray were isolated and gene expression profiles were analyzed. MIAPaCa-2 cells were irradiated with proton or x-ray with 2 Gy or 8 Gy. After 3 h or 12 h of irradiation, RNA was isolated and gene expression profile was examined by DNA microarray for a total of 8 specific conditions (n=1) plus control (n=1).