Hypothesis-testing of multiple (polyphyletic) vs. single (monophyletic) origin of RIP mutation-associated haplotypes of <i>Leptosphaeria maculans</i>.

Kishino–Hasegawa tests were used to assess different hypotheses of RIP evolution by comparing tree topologies as implemented in PAUP*. The probabilities (P) of obtaining better trees were assessed using two-tailed tests, the full optimization criterion and 1000 bootstrap replicates. aTree scores refer to branch lengths for the tree topologies. For the maximum likelihood analysis, tree scores are given as –ln of branch length. b‘Best’ refers to the ‘shortest’ tree, which is likely to be the most accurate.