Epigenomic profiling by optimized ChIL-seq for tissue

We optimized the epigenomic profiling technology ChIL-seq for tissue. We confirmed the "ChIL for tissue" has sufficient sensitivity, specificity and reproducibility, in identification of enhancers, transcription factors, transcriptional activation genes in a single-thin-section of tissues. Overall design: The targets of ChIL-seq are the transcription factor: Hnf4alpha, histone modification: H3K27ac and RNA Polymerase II. The tissues were profiled using single cryo-sections from human breast cancer, mouse liver, heart, testis, and cardiotoxin-injured muscle for regeneration study. The RNA-seq data of the tissues were obtained.