Genome wide analysis of the effect of MMP13-ablation on mouse dental pulp tissue

Tissue form 3-month old mouse molar teeth was used to obtain mRNA profiles in developing wild-type (WT) and matrix metalloproteinase-13 (Mmp13-/-) mice using high throughput RNA-seq. Samples were analysed in triplicate using Illumina HiSeq 2500 system. Sequence reads that passed stringent quality filters were analyzed at the transcript isoform level using after passing a t-test (P < 0.05), post-hoc test (Storey with Bootstrapping) with a corrected q value of 0.05. qRT-PCR validation was performed using SYBR Green assays. the effect of Mmp13 on tooth development and mineralsiation processes was the focus of this work. Dental pulp mRNA profiles of 3-month old wild type (WT) and Mmp13-/- mice. 3 Biological replicates per genotype.本研究采集3月龄小鼠磨牙组织，采用高通量RNA测序（RNA-seq）技术，获取发育阶段野生型（wild-type, WT）及基质金属蛋白酶-13敲除（matrix metalloproteinase-13, Mmp13-/-）小鼠的mRNA表达谱。所有样本均通过Illumina HiSeq 2500测序系统完成三次生物学重复分析。经严格质量过滤的测序reads将在转录本异构体水平进行分析，分析前需通过t检验（P < 0.05）及事后检验（采用Bootstrap法校正的Storey检验），校正后的q值阈值设为0.05。本研究采用SYBR Green检测法完成qRT-PCR验证实验，核心聚焦于基质金属蛋白酶-13（Mmp13）对牙齿发育及矿化过程的调控作用。本数据集包含3月龄野生型（WT）及Mmp13-/-小鼠的牙髓mRNA表达谱，每种基因型设置3个生物学重复样本。